western blotting method for diagnosis of hydatid cyst antigens

Detection of specific antigens of Newcastle disease virus using an absorbed Western blotting method

Newcastle disease virus (NDV) is an economically important poultry pathogen with a worldwide distribution that may infect a wide range of domestic and wild avian species. The identification of different pathotypes of NDVs plays an important role in the diagnosis and development of vaccines to control and eradicate NDV infections. In our previous study, we showed that mono-specific antibodies ca...

evaluation of different antigens in western blotting technique for the diagnosis of sheep haemonchosis

background: to use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. methods: peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of hae­monchus contortus were determined using sds-page and western blotting. five lambs were infected with 10,000 third stage larv...

immunological diagnosis of human hydatid cyst using western immunoblotting technique

background: echinococcosis is a parasitic disease with worldwide distribution which is caused by the tapeworms echinococcus granulosus . diagnosis of the disease relies on imaging techniques, but the techniques are not able to differentiate the cyst from benign or malignant tumors; hence, appropriate serologic methods are required for the differential diagnosis of the infection. materials and m...

Diagnosis of Hydatid Cyst of Ventriculars\' Septum by Echocardiography

A 31 year old man, admitted because of acute onset of fever and chills as well as anorexia and weakness. Positive findings included an ejection systolic murmur a W.B.C of 15200 with Eosino­phillia;a R.B.B.B. Pattern in E.C.G., a lobulated mass in upper part of the septum in Echocardio­gram. Patient underwent cardiac surgery with tricuspid replacement, and diagnosis of Hydatid cyst was confirmed. 

Specific recognition of hydatid cyst antigens by serum IgG, IgE, and IgA using western blot.

Diagnosis of hydatid disease in humans relies on the detection of specific antibodies against antigens of the metacestode from Echinococcus granulosus. The specificity and sensitivity of current immunological techniques based on specific serum IgG rely on the way antigens are purified. We used Western immunoblotting to detect specific IgG, IgE, and IgA antibodies in serum from patients with hyd...

Preparation of Semi-purified Laminated Layer of Hydatid Cyst for Diagnosis of Infection